Journal: iScience
Article Title: Portable wide-field femtoliter-chamber imaging system for point-of-care digital bioanalysis
doi: 10.1016/j.isci.2024.110868
Figure Lengend Snippet: Digital counting of viral RNAs (A) Number of positive chambers obtained with COWFISH2 (red), COWFISH (blue) or confocal microscopy (green). The linear regressions are represented by solid lines. The values of the blank mean +3 SD are shown as dotted lines ( n = 3 technical replicates). (B) Number of positive chambers obtained using COWFISH2 for SARS-CoV-2 (red), FluA (blue), or FluB (green). The linear regressions are represented by solid lines. The values of the blank mean + 3 SD are shown as dotted lines ( n = 3 technical replicates). (C) Number of positive chambers obtained using crRNA for SARS-CoV-2 (red), FluA (blue) or FluB (green). The concentration of tgRNA was 300 fM. Asterisks represent the significant differences determined by unpaired two-tailed Student’s t test with equal variance (∗∗: p < 0.01, ∗∗∗: p < 0.001, and ∗∗∗∗: p < 0.0001).
Article Snippet: crRNA for FluB , GeneDesign , N/A.
Techniques: Confocal Microscopy, Concentration Assay, Two Tailed Test